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Among these 7 specimens were non typeable (i.e., neither a G nor a P genotype could be identified) by multiplex-PCR.
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Extractions of single specimens were non-destructive, using the DNeasy Blood & Tissue Kit (Qiagen GmbH, Hilden, Germany).
By histology alone, 27 of the remaining 121 ex vivo core needle biopsy specimens were non-diagnostic (22%).
In cases in which multiple individuals were available from the same colony, entire or partial specimens were destructively extracted; in cases in which only a single or a few specimens were available, specimens were non-destructively extracted.
Of the 96 RCC specimens, 83 were clear cell carcinoma and the remaining 13 specimens were non-clear cell carcinoma (3 papillary carcinoma, 3 chromophobe carcinoma, 3 sarcomatoid carcinoma, 2 granular carcinoma and 2 collecting duct carcinoma).
A full list of collectors and localities is given in Additional file 1. Extractions of single specimens were non-destructive, using a standard phenol-chloroform method or the DNeasy Tissue Kit (Qiagen GmbH, Hilden, Germany).
Impacted and not impacted specimens are non-destructively evaluated with lock-in thermography (LT).
Moreover, up to 15% of specimens are non-reportable even when testing samples with plasma HIV-RNA > 1000 copies/mL.
This is considered to be because of the fact that a considerable proportion of breast cancer specimens is non-cancerous and that the levels of virus are low in breast cancer.
This method also overcomes some of the limitations of SEM imaging in that the embryos never have to be dehydrated, can be kept at room temperature in fixative indefinitely, can be used on museum specimens, is non-destructive (unlike SEM), and also provides simultaneous tissue resolution below the differentiating skin.
Specimens were from non-fever, non-rash ill patients less than 35 years old.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com