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Specimens were next measured for quantitative protein expression with a DAB filter.
Specimens were next washed with PBS, incubated O/N with the secondary antibody, washed for 2 h with PBS, dehydrated and cleared.
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The tissue specimens were washed the next day and were then dehydrated by graded ethanol series.
No living specimens were seen for the next 10 years, although commercial harvests later resumed.
Specimens were obtained with the next scheduled blood draw after the fever, and exclusion criteria were minimized.
There are only a few historical reports of Desmognathus on the Ozark Plateau [ 25, 26], but no credence has been given to these records [ 27, 28] since no museum vouchers exist and no subsequent specimens were found for the next 40+ years.
Specimens were instrumented with the ProTaper Next or New One Shape.
On the next day, specimens were dewaxed in xylene and then rehydrated in a descending alcohol series.
On the next day, specimens were dewaxed in two changes of fresh xylene and then rehydrated in a descending alcohol series.
According to genetic data, most shallow water Oncholaimus specimens were placed in a clade next to Viscosia; the primary shallow-water clades containing the majority of Oncholaimus and Viscosia specimens isolated in this study are denoted by a black square in Additional file 2, Figure S1.
The next oldest Oryza specimens were collected in 1797 by Ledru in Puerto Rico and also in the Virgin Island formerly known as St Thomas during the same trip [42].
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