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To determine the geometrical quality, the internal and the external surfaces of the specimens were mapped.
Less than half of specimens were mapped under the SDTF biome in all of the maps.
Large proportions of specimens were mapped under other biomes, mainly the Chaco and Cerrado, or under neighbouring biomes in the Andes.
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The temperature profiles along the length of heater and specimen are mapped by using an infrared microscope (Quantum Focus Instruments, MWIR-512 InSb IR FPA camera) with 0.1 K temperature and 2 μm spatial resolution.
In the 1097 fecal specimens, taxa were mapped to 261 bacterial families, 626 genera, and 844 species.
All the spectra obtained from distinct calcified regions without surrounding tissue and/or embedding material, from the five specimens that were mapped with FTIR-RM (Table 1), contained PO4 peaks typical of apatite and similar to that of bone; carbonate peaks similar to those present in bone; and small amide peaks (of proteins and/or collagen) with much lower intensity than in bone (Table 2).
For the remaining C. fiber specimens, sequencing reads were mapped onto the assembled C. fiber mitochondrial genome using runMapping (454 software) and the iterative mapping tool IMA [46].
Regions such as these in the specimens with island morphology that were mapped with FTIR-RM (Calc1, Calc2 and Calc8) were composed of amides that are the main functional groups of all proteins and soft tissues.
SEM/SDD-EDS performed in the scanning electron microscope (SEM) was carried out using the Bruker QUAD SDD (a cluster of four 10 mm2 SDDs; Berlin, Germany) on the same polished surfaces of specimens Calc1 and Calc2 that were mapped also with FTIR-RM and qBSE, to spatially map the relative concentrations of the chemical elements that are present in these deposits.
In addition, normal areas were mapped for six cystoprostatectomy specimens obtained from patients undergoing treatment for bladder cancer.
Osteons (n = 12,690) were mapped within microradiographs of femoral mid-diaphyseal specimens (n = 88; 45 male, 43 female; 17 97 yrs).
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