Sentence examples for specimens were manually from inspiring English sources

Next, the specimens were manually polished using 600, 1200 and 2000 grit sandpaper (Norton S.A., São Paulo, SP, Brazil) and subjected to hardness test using a Hardness Tester HMV 2000 (Shimadzu, Tokyo, Japan) at automatic procedure with a load of 50 g applied for 15 s.

To investigate 19 bladder tumours in aCGH analysis, tumour specimens were manually microdissected and DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Hilden/Germany) according to manufacturer's protocol.

8 Briefly, muscle biopsy specimens were manually homogenized in phosphate-buffered saline containing 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1% protease inhibitor cocktail, and 1% phosphatase inhibitor cocktail.

Fifty seven fields per patient, including demographics, preoperative prostate-specific antigen, pathological Gleason score and stage and other pathological data relating to the prostatectomy specimens were manually entered in a non-relational database (Microsoft Excel spreadsheet) for the first 853 of these cases (2004 2008).

Moreover, because a specimen stays inside a single magnetic shield case during the course of repeated measurements and thermal demagnetizations, spurious magnetization is hardly acquired, unlike when the specimen is manually handled outside the shield case.

The first 200 specimens encountered were manually separated and mounted on cardboard slides in a standard orientation (edge on, aperture facing).

Venoms from captive specimens were collected manually by milking.

Specimens were dissociated manually in PBS− and then observed under an MZ16F fluorescence stereomicroscope (Leica).

For preliminary analysis, the 12 sequences obtained from Alloteropsis specimens were aligned manually with 137 of the 156 ndhF sequences used by Aliscioni et al. (2003).

After that, specimens were finished manually on 600, 1000, and 1200-grit sandpaperspers with running water, for 10 seconds on each side [ 14].

The specimens were pulverized manually in liquid nitrogen; aliquots of the tissue powders (about 100 mg each) were processed to extract DNA and total RNA separately, following conventional phenol-chloroform protocols.