The number of specimens was limited due to financial reasons.
The maximum length of the specimens is limited to 90 mm.
However, when using a conventional scanning electron microscope, the sample chamber must be kept dry and under high vacuum during use, so the specimens are limited to nonvolatile, conductive materials (Tsuda et al. 2011b).
The specimens are limited to distinct layers formed during transgressive phases that opened inner and partly restricted platform areas to open-marine and more distal (deeper subtidal) environments, possibly accompanied by a transition from a rather arid to more humid climatic conditions.
Experimental studies on the infectiousness of lesion crusts, specifically in preserved specimens, are limited.
However, evidence concerning NAATs accuracy for non-respiratory specimens are limited.
Moreover, evidence concerning NAATs accuracy for non-respiratory specimens are limited and further research is needed before NAATs can be recommended for the diagnosis of TB in children who cannot produce sputum [ 12, 15].
Since MBs/PNETs are rare tumours and the availability of the specimens was limited to 39, the positively stained tumours (scores 1-3) were pooled for most statistical analyses, including the studies on patient survival.
However, the analysis of proteins in tissue specimens is limited to the semi-quantitative immunohistochemistry (IHC) assay that are required to obtain the tissue spatial information and cell type-specific staining patterns.
Furthermore, similar studies can be done to understand the behaviour of potential barcode loci in other large taxonomic groups; at the moment, however, the availability of whole mt genomes from multiple conspecific specimens is limited in most animal groups.
