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After demoulding, specimens were lightly rubbed with an abrasive pad to remove laitance and then cured in the same conditions as rectangular prisms.
After immunohistochemistry, the specimens were lightly counterstained with 10% hematoxylin, dehydrated, and mounted.
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Using Nomarski optics, we show that in normal control specimens, astrocytes were lightly immunostained and presented with abundant short thin branches and a lace-like pattern that formed rounded arborizations.
The specimens were then lightly counterstained with Mayer's hematoxylin, dehydrated, and xylene-based mounted under glass cover slips.
Thirty specimens of eight-week-old male rats were lightly anesthetized.
Every two days, the animals were lightly anesthetized with 40 mg/kg ketamine and nasal wash specimens were collected in 1 mL sterile phosphate-buffered saline as described previously.
All sections were lightly counterstained with haematoxylin.
Sections were lightly counterstained with hematoxylin.
Slides were lightly counterstained with H&E.
The nuclei were lightly counterstained with hematoxylin.
Mice were lightly anesthetized with halothane.
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