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Adult specimens were isolated from sites of infection (skin, osteoarticular, and blood infections) of patients admitted to hospitals in various regions of France from 2002 to 2008.
Nematode specimens were isolated from their host animals (C.
injection, mice were sacrificed and synovial specimens were isolated.
Tibia and liver specimens were isolated and processed for histological examination.
Nuclei from liver specimens were isolated on saccharose gradient, according to Hogeboom [ 25].
Rat lumbar disc specimens were isolated using a method reported earlier by Ponnappan et al. [ 8].
Similar(31)
RNA from clinical specimens was isolated using RNA-Bee (Campro Scientific, Berlin, Germany).
RNA from the EAC patient biopsy specimens was isolated performing the TRiZol Isolation method as described previously [26].
DNA from tumor specimens was isolated by phenol-chloroform extraction.
Total RNA from all 54 tissue specimens was isolated using Trizol kit (GIBCO BRL Life Technologies).
DNA from tumor specimens was isolated applying the QIAamp® DNA Mini Kit (Qiagen, Hilden, Germany).
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