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Based on their morphological characters, the specimens were initially identified as two different species.
All the specimens were initially austenitized at 927°C (1700°F) for 2 h.
These specimens were initially identified as two species (G. pacifica Tanaka and G. filamentosa Chou ex Taylor) based on their morphological features.
In order to evaluate the residual seismic capacity of CFRP-confined columns, three of the confined specimens were initially loaded to induce damage.
The 12 cadaver specimens were initially intended to form 3 equal groups, but the challenges faced with MRI settings required the use of an additional specimen, which resulted in unequal group sizes.
Seventeen specimens were initially heated to six various preselected temperatures up to 1000 °C and were subsequently cooled down to ambient temperature via two different methods: air cooling and water cooling.
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Detection of serotype-specific sequences of pneumococcus in CSF specimens was initially standardized and validated by using the culture positive specimens, keeping the respective isolate as control.
Owing to these circumstances, cultures from human brain tumour specimens are initially overgrown by normal cells, for example, endothelial cells, fibroblasts and other nonmalignant cells.
Specimens are initially screened for influenza A and B viruses by real-time reverse transcription PCR (rRT-PCR); samples positive for influenza A are then subtyped as H1N1, H3N2, H5N1, or H7N9.
Additionally and importantly, improvements in reagents available for storage and purification of RNA from clinical blood samples should also enhance quality of specimens, especially in cases where specimens are initially harvested at multiple clinical sites; the recently introduced PAXgene blood RNA stabilization reagent is an example of such improvement [ 31].
The HPV L1 gene DNA in liquid-based Pap cytology specimens was initially amplified by the degenerate MY09/MY11 PCR primers and then re-amplified by the nested GP5+/GP6+ primers, or the heminested GP6/MY11, heminested GP5/MY09 primers or their modified equivalent without sample purification or DNA extraction.
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