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The specimens were individually positioned perpendicularly to the tester tip, and the VHN values were recorded by a software program (CAMSTM_WIN program - Newage Testing Instruments, Southampton, PA, USA).
In groups G2, G3 and G4, the specimens were individually coupled to a metallic circular matrix and polished with SiC sandpaper (#1200) for 5 s to remove the outer resin layer, obtain a standardized and stable surface.
Specimens were individually heparinized (Sigma Aldrich, H4784) by intraperitoneal injection (50 µl/specimen, 1000 U/ml) and anesthetized by isoflurane inhalation (Attane, 83642) according to approved institutional protocols.
All specimens were individually barcoded, photographed and measured for body-length.
Collected mite specimens were individually rehydrated for two hours in 200 μl of sterile water before being crushed in liquid nitrogen.
Standard COI barcodes for all specimens were individually Sanger sequenced and compared against the Barcode of Life Data Systems for taxonomic confirmation.
Similar(51)
From the linear portion of the curves the compliance (C) due to all specimens is individually calculated and the compliance is plotted against the normalized crack length, i.e., a/W.
The specimen were individually placed on Franz-type diffusion cells and clamped between the donor and receptor compartments.
To prevent the influences of other specimens, each specimen was individually put into a cup with a different combination of salinity and temperature.
First, when we analyze the three Neandertal bones Vi33.16, Vi33.25, and Vi33.26 separately, we obtain consistent values of the D statistics, which is unlikely to arise under the hypothesis of contamination because each specimen was individually handled and was thus unlikely to have been affected by the same degree of contamination (SOM Text 15).
Briefly, tpsRelw uses a generalized Procrustes analysis (GPA) to compute a consensus shape with a minimum sum of squared Procrustes distances to other specimens wherein each specimen is individually superimposed onto the average of previous specimens until all specimens are averaged.
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