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All specimens were fixed in paraformaldehyde and embedded in paraffin.
Specimens were fixed again overnight at 4 °C.
In each group, six specimens were fixed with a 3.5-mm locked proximal tibia plate and six specimens were fixed with a 4.5-mm locking plate.
Muscles from 10 fresh cadaver specimens were fixed by high-pressure perfusion using buffered formaldehyde.
The specimens were fixed in 10% buffered formalin for 1 to 2 weeks.
The specimens were fixed to the microscope on a metal support using adhesive tape.
The specimens were fixed with ethylene glycol for 30 min and dried at 50°C.
All specimens were fixed using one 7.0-mm iliosacral screw into the S1 body.
Specimens were fixed in 10%% formaldehyde for at least 24 h.
The test wire specimens were fixed on the bracket with elastomeric ligature (Morelli, Sorocaba, Brazil).
Some specimens were fixed in 10% neutral formalin for 3 days and prepared to paraffin embedding.
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