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After dehydration through gradient ethanol solutions, the specimens were embedded in Araldite-Epon (Embed-812, Electron Microscopy Sciences, USA).
The tissue specimens were embedded in araldite.
Dehydrated specimens were embedded in epoxy resin.
Specimens were embedded in paraffin, and tissue slices (5 μm in thickness) were prepared.
Finally, the specimens were embedded in transparent chemically polymerized methyl methacrylate resin (methyl methacrylate 99%, Sigma-Aldrich, Steinheim, Germany).
After fixation with 10%% phosphate-buffered formalin for at least 24 h, specimens were embedded within paraffin and sectioned.
Following dehydration by graded ethanol series specimens were embedded in polymethylmethacrylate.
All lung specimens were embedded in paraffin and stained with hematoxylene and eosin.
After dehydration in ascending concentrations of ethanol and xylene, the specimens were embedded in paraffin.
After dehydration, the LG specimens were embedded in paraffin, cross-sectioned, and stained.
After being treated with 30% sucrose, specimens were embedded in OCT.
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