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Then, specimens were digested with 32.5% HNO3 (Merck, Germany).
After the CVL specimens were digested with Proteinase K, 10 µL of each cell digest was taken to detect HPV DNA using the L1 MY09/MY11 modified PCR system with AmpliTaq Gold polymerase as described elsewhere [18].
After the pretreatment, the specimens were digested with acidic pepsin solution.
The 16 S rDNA 545 bp amplicons obtained by PCR from biopsy specimens were digested with H inf I (biolab – New England) according to the manufacturer's instructions.
ASCs were harvested from the adipose tissue of one 6-month-old Ovis aries weighing 37.4785 lb, and 0.5 g adipose tissue biopsy specimens were digested with 800 µl collagenase I (180 U/ml) solution using the protocol of Dubois and colleagues [ 20].
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Briefly, the cartilage specimens were digested overnight in Dulbecco's modified Eagle's medium: Nutrient Mixture F-12 (F12/DMEM) with 5% fetal bovine serum (FBS) and 0.08 mg/mL collagenase type II (Invitrogen, Grand Island, NY, USA).
Whole blood specimens were digested using methods from Csanaky et al. (2003).
Specimens were digested and decontaminated using the Nalc-NaOH method, and resuspended in 2.0 mL of phosphate buffer.
The sputum specimens were digested, examined microscopically for acid-fast bacilli, and inoculated into Löwenstein-Jensen slants by standard procedures.
In a similar fashion, whole-blood specimens were digested according to method of Csanaky and Gregus (2003).
Sputum, gastric aspirate, and urine specimens were digested and decontaminated by the N-acetyl-L-cysteine (NALC -NaOH method.
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