After demineralisation, the specimens were dehydrated through an ascending ethanol series and mounted in paraffin.
Fixed specimens were dehydrated in graded ethanol solution with serial concentrations of 50%, 70 %, 80 %, 90 and 100%.
The specimens were dehydrated by a graded series of ethanol (75, 85, 95, and 100%) and embedded in Epon 812.
The fixed specimens were dehydrated in an ascending grade of ethanol, cleared in benzene, and embedded in paraffin.
The fixed specimens were dehydrated using 50, 70, 80, 90, 95 and 100% (v/v) graded ethanol, and transferred into t-butyl alcohol.
Specimens were dehydrated in ascending series of alcohol rinses and embedded in a glycol methacrylate resin (Technovit 7200 VLC; Kulzer, Wehrheim, Germany).
All the specimens were dehydrated in increasing alcohol concentration (25, 50, 75, 100%) and immersed in HMDS (Hexamethyldisilazane) for 10 min [15].
Prior to scanning electron microscopic observations, the HAM specimens were dehydrated in a graded ethanol series of 25, 50, 75, 95 and 100%% each for 10 min.
Fixed specimens were dehydrated in a graded acetone series and embedded in Spurr's resin according to the method of Lin et al. (2012).
The specimens were dehydrated through a graded alcohol series.
Specimens were dehydrated in increasing concentrations of alcohol and embedded in EPON 812.
