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The insulation arrangements within the concrete specimens were confirmed using X-ray computed tomography (CT) without damaging the specimen.
The identity of the sequences and specimens were confirmed using the MegaBLAST search function in GenBank.
Positive specimens were confirmed using Enzygnost Anti-HIV 1/2 Plus ELISA (Dade Behring, Marburg, Germany) and discrepant results were resolved by Western Blot.
In 2005 dried blood spot specimens were tested for HIV antibodies using GACELISA, reactive specimens were confirmed using an anti-HIV GACPAT immunoassay, with confirmatory testing conducted on discordant results using the HIV Blot 2.2 Western Blot assay (AbbotMurex) [ 32, 33].
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Specimens detected to be RPR positive were confirmed using a Treponema pallidum particle test (TPPA, Serodia, Japan).
Biliary stone cases were confirmed using abdominal ultrasound, endoscopic retrograde cholangiopancreatography, medical or surgical records, or pathologic specimens for those who underwent a cholecystectomy.
These observations were confirmed using pharmacologic inhibitors.
Results were confirmed using qPCR analysis.
Antibody specificity was confirmed using human skin specimens for SCCA and colonic cancer specimens for Ki67, as positive controls.
Specificity of detection was confirmed using no probe staining and RNase-digested specimen.
This was confirmed using TBR1 null mice.
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