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Exact(8)
All specimens were cleared in graded glycerol for 2 5 days.
The specimens were cleared in xylene (20 30 times).
Specimens were cleared in xylene and embedded in paraffin at 56 °C in a hot oven for 24 h.
All specimens were cleared and stained with alizarin red S [ 42] in order to display the osseous skeleton.
The tissue specimens were cleared in xylene, embedded in paraffin, sectioned at 4 6 μ thickness, stained with Haematoxylin-Eosin (H and E) [ 22].
Specimens were cleared by centrifugation according to standard methods, and were labelled with a study number such that all analysis was blinded.
Similar(52)
They were then digested in a trypsin solution (1% in 30% saturated sodium borate solution, renewed when blue) until the specimen is cleared.
Although the main specimen was cleared, some final margins were persistently positive because of disease identified at the edge of the additional tissue resected (phase III, branches C2 and D2) in 8 and 22 cases for the control and device arms, respectively.
All saliva specimens were clear on inspection.
The need for a simple genotyping system, effective in clinical specimens, is clear.
Specimens of flank skin were cleared by dehydration in a graded series of glycerin solutions (50 100%) and photographed using a Wild M400 Stereomicroscope and SPOT Insight digital camera.
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