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Specimens were again centrifuged at 15 000 r.p.m. for 2 min at 4°C.
Efforts to culture the organism from biopsy specimens were again unsuccessful (different solid and liquid media, blood or chocolate agar, guinea pig, Balb/c mice).
Specimens were again washed with PBS, incubated with peroxidase-labeled streptavidin (DAB; Dako Code K3468) for measurement of the intensity of immunoreactivity.
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The only thing standing in the way of commercial plastination of museum specimens is, again, lack of facilities and people to perform the procedure.
All patients were subjected to Patey's modified radical mastectomy three weeks after the last cycle and the specimen were again subjected to immuno-histochemistry to evaluate for any change in the Bcl-2/Bax ratio and for the histological tumor size, margins.
After the analysis of cox1 sequences, every specimen was again inspected by a taxonomist to correct possible misidentifications and, in many cases, to improve the existing taxonomy by a more detailed comparison of male genitalia and other structures.
Thereafter, the specimen was again centrifuged (400 g, 1 min).
After the specimen was elongated 30%35%%, which is well within the linear elastic region [ 1], the deformed specimen was again imaged.
After that, each specimen was again stored in the storage vessel until the 15 min of this interval of low pressure was completed (time point 90 min in Fig. 3).
Specimens and antibodies were again pre-blocked for one hour at 4°C.
Specimens were fixed again overnight at 4 °C.
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