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Having located potential hybrid specimens, we use morphological criteria, coupled with knowledge of intra- and interspecific Heliconius genetics [ 72, 83, 88- 93] to decide whether they constitute hybrids or intraspecific variants.
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As environmental specimens we used an agricultural sandy soil (arable sandy loam, ASL), and white quartz sand (Sigma-Aldrich).
In order to guarantee comparability between specimens we used only skins of adult male specimens in spring or summer plumage collected from breeding areas.
To amplify the GFP signal in some specimens we used a mouse monoclonal antibody to GFP (#A-11120; Molecular Probes, Leiden, Netherlands) at 1∶1000.
Details of the specimens we used are given in the Table S2, along with the sources for estimates of stature, mass, and long bone lengths.
In addition, the breast tissue images used in [12] show distinctive blue and red/pink stains in their Hematoxylin and Eosin (H&E) images, which however do not apply to the lung tissue specimens we used.
Instead, for clinical specimens, we used a newly designed primer, 120-M36.
To determine the protein expression of RRM2 in the tissue specimens, we used IHC techniques on FFPE tissues.
To estimate quantitatively their representation in our wholemount and section specimens, we used general and specific immunohistochemical markers.
For screening of serum specimens we used the SARS-CoV ELISA kit (EUROIMMUN AG, Lübeck, Germany) with minor modifications.
Of the 14 Rattus specimens we used for the whole mitochondrial genomes, 11 were from homogeneous clades.
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