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Finally, most morning urine specimens we obtained were first spot and small numbers of them were second spot specimens.
To validate these observations in clinical specimens, we obtained tumor samples from eight GBM patients before and after bevacizumab treatment (see Supplementary Table 1 for patient characteristics).
For 79 specimens we obtained good sequences for all three genes and these form the nSSU*, nLSU* and COX1* datasets analysed below.
It is recognized not only by the specimens we obtained in 2002, but also by 63 specimens collected in 1998 by the researchers from Chengdu Institute of Biology, Chinese Academy of Sciences (Fei and Jiang, personal communication).
To determine whether these antibodies may be useful as markers of neuronal apoptosis and axon degeneration in human neuropathological specimens, we obtained autopsy brain tissue from five patients who had been diagnosed with acute or subacute infarcts of the CNS (Table 1).
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Since the majority of the surgical specimens we obtain come from grade III and grade IV discs, there were fewer grade I and grade V discs in the present study.
Counting the number of DBS specimens classified as recently HIV-infected by the cBED assay in the sample of all individuals who had a previous positive HIV test more than 306 days before the date of the cBED assay-tested specimen, we obtained a long-term FPR of 0.0169 (95% CI 0.0100 0.0266).
For each specimen we obtained multiple strands that were assembled in the program ABI PRISM Sequence Navigator version 1.0.1 (Applied Biosystems).
For a subsample of specimens, we also obtained partial sequences of two additional mtDNA markers: the NADH dehydrogenase subunit 2 (ND2, 1160 bp) and 5 (ND5, 1132 bp) (Additional file 1: Table S2).
For the specimens from adults, we obtained written consent to revearch use by their next of kin.
Because urine specimens were not available, we obtained 112 serum specimens of patients with KD, collected as part of the recent Paediatric Heart Network study, and compared them to patients initially suspected to have KD but ultimately diagnosed with non-KD febrile illnesses (Fig 2E and F).
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