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Although the used specimen was collected relatively recently and kept in a DNA-friendly preservative (not formalin as frequently used with old museum specimens), we believe that the exclusion of PCRs as facilitated by NGS (Next Generation Sequencing) removes a great obstacle in DNA sequencing of collection specimens.
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The 25G FNB needle is a smaller-gauge needle and has a side bevel for the acquisition of core specimens; we believed that its smaller size would allow greater flexibility in puncturing tissue when the tip of the echoendoscope was completely angulated and that the side bevel could obtain core specimens, which could improve the diagnostic yield of targeted lesions.
There are several possible explanations for the S. pneumoniae 16S rDNA heterogeneity in this specimen (see above); however, we believe that at least some of the heterogeneity is best explained by the presence of two (or more) S. pneumoniae strains.
This study does not evaluate the availability of such controls but, given the high prevalence of non-cancer specimens in our collective archives, we believe that there will be several different sources of normal tissues.
In sum, we believe that specimen-based natural history data represent a powerful platform for reforming undergraduate instruction in biology.
We believe, however, that specimens collected in the 1970s and 1980s attributed to P. canaliculata (see below) are P. haustrum.
Therefore, we believe that whenever possible, hysterectomy specimen should be used for DNA ploidy analysis.
We believe the major impact was associated with specimens containing a sub-optimal cell content (20%).
However, as all the specimens were prepared and tested in a uniform and reproducible manner, we believe that these results provide useful information.
We believe our results show that urine is appropriate, and is a useful but minimally invasive specimen for such studies.
We believe".
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