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The methodology used for RNA extraction from tissue specimens was that previously described for RNA extraction from cell culture.
Another feature in gliotic brain specimens was that FABP7 was weakly positive in the cytoplasm of some neurons (data not shown).
The basis for disregarding the results for these specimens was that the loadings for both specimens were not concentric.
One reason for the lack of sales, and for the poor condition of many surviving specimens, was that while the fair was open, the economic Panic of 1893 began, one of the worst depressions in the nation's history fifty cents could make the difference between a family eating or starving at a time when the average visitor to the fair spent $1.18.
A curious observation in the specimens was that the 18 mesenteries are aligned as in pair and most adjoining mesenteries have fused medially and aborally displaying Y-shaped sections in the micro-CT analysis (Movies S1, 3), which is reflected to the oral views (Figure 1B, D, F).
The primary reason given by hospital pathology departments for not sending specimens was that they had been destroyed or were no longer available (35%) [ 29, 30].
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The reason why high-density FGLNAs were not observed on these high initial stress specimens is that the relatively low surface roughness may lack enough surface area to further enhance the growth of FGLNAs on the specimens.
But perhaps the most distinguished of the specimens is that of Broca, a 19th-century physician and anthropologist who was the father of modern brain surgery.
Another possible explanation of low or unmatching results for C. batrachus specimens is that geographically divergent populations of C. batrachus may exist.
A potential problem with the application of microarray technology to primary human tumour specimens is that the specimens may represent mixtures of cell types.
But what's most remarkable about this specimen is that the feathers contain traces of their original colours, which means that we can reconstruct the coloration of this extinct lineage of early birds.
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