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Additional information about these specimens was provided previously.[28] Total RNA was isolated as described above and cDNA was synthesized using MMLV-reverse transcriptase kit and Oligo dT 12 18 primer (Invitrogen), according to manufacturer's protocol.
Initial evidence of WNV in the specimens was provided by nested PCR amplicons co-migrating with the WNV positive control.
Support for the collection of research specimens was provided by the National Oceanographic and Atmospheric Administration (NOAA; United States), NOAA's National Undersea Research Program, and UNCW's Coastal Oceans Research and Monitoring Program.
Among the 172 patients who underwent standard treatment, informed consent to use specimens was provided from 167 patients, for whom the median follow up term was 55 months (range 11 122 months).
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Special rooms equipped for teaching and for handling specimens are provided in many museums.
Molluscum contagiosum specimens were provided by a dermatologist.
C. semilaevis (130 145 g) specimens were provided by the Tianjin Haifa Aquaculture Center.
Human tissue specimens were provided by the Department of Pathology at University of California, Los Angeles (UCLA) Medical Center under an approved UCLA Institutional Review Board protocol.
To support this practice, a method to convert Jc obtained with other sized CT specimens is provided in ASTM E1921.
Two of the twelve tendons in the specimens were provided in the NonlinearBeamColumn elements representing the bottom flange of the specimens.
In this paper chromatic variability of all the collected specimens were provided and the accuracy of initial morphological identification was discussed.
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