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During testing, the hydration state of the specimens was maintained by misting (Pariboy; Pari GmbH, Starnberg, Germany) and running deionized water over the specimen (Perfusor® Compact S, B BRAUN, Melsungen, Germany).
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or thermal exposure, it is imperative to insure environmental protection during the entire transfer process so that all specimens are maintained in a pristine state before, during, and after analysis.
Specimens are maintained in liquid nitrogen until analysis.
The specimens were maintained in aerated tanks for two weeks.
The remaining specimens were maintained live, sexed and used as founders for further experimentation as described below.
Specimens were maintained at 25°C via a dual stage and objective heating unit (Tempcontrol 37-2 digital; Zeiss).
Fresh frozen human vertebral specimens were maintained at −20°C until approximately 24 hours prior to testing.
Specimens were maintained at room temperature and processed within 72 h after collection.
All tissue specimens were maintained at the Department of Pathology and patient medical records were available at Baystate Cancer Center.
All biological specimens are maintained through Freezerworks™, a biospecimen management software, and stored locally.
Blattarian specimens were maintained in the dark at 30 ±1°C and 60-70% RH prior to RNA extraction.
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