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The specimens was fixed in 10% formalin for 24 h and embedded in paraffin.
As discussed in the 'Theoretical aspects' section, the maximum stress in fatigue cycles applied over such specimens was fixed at 97.45 MPa.
One part of the specimens was fixed in 4% buffered formalin and embedded in paraffin.
The proximal 3/5 of the specimens was fixed in 4% PFA for routine histology analysis.
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Kidney specimens were fixed in 0.1 mol/L phosphate-buffered Karnovsky's fixative.
Specimens were fixed again overnight at 4 °C.
All specimens were fixed in paraformaldehyde and embedded in paraffin.
In each group, six specimens were fixed with a 3.5-mm locked proximal tibia plate and six specimens were fixed with a 4.5-mm locking plate.
Muscles from 10 fresh cadaver specimens were fixed by high-pressure perfusion using buffered formaldehyde.
The specimens were fixed in 10% buffered formalin for 1 to 2 weeks.
Specimens were fixed in 4% paraformaldehyde.
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