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To allow the release of AFB1-lys from albumin, protein in serum specimens was digested in the presence of stable-isotopically labelled internal standard (H4-AFB1-lys) in 4 hours at 37°C by the use of a commercially available mixture of proteinases (Pronase™).
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Briefly, subchondral bone specimens were digested by sequential collagenase type I digestion, followed by cell culture in BGJb medium containing 20% FBS.
Then, specimens were digested with 32.5% HNO3 (Merck, Germany).
After the CVL specimens were digested with Proteinase K, 10 µL of each cell digest was taken to detect HPV DNA using the L1 MY09/MY11 modified PCR system with AmpliTaq Gold polymerase as described elsewhere [18].
Whole blood specimens were digested using methods from Csanaky et al. (2003).
After the pretreatment, the specimens were digested with acidic pepsin solution.
Specimens were digested and decontaminated using the Nalc-NaOH method, and resuspended in 2.0 mL of phosphate buffer.
The sputum specimens were digested, examined microscopically for acid-fast bacilli, and inoculated into Löwenstein-Jensen slants by standard procedures.
In a similar fashion, whole-blood specimens were digested according to method of Csanaky and Gregus (2003).
Sputum, gastric aspirate, and urine specimens were digested and decontaminated by the N-acetyl-L-cysteine (NALC -NaOH method.
Specimens were digested and decontaminated by the N-acetyl-L-cystein-sodium hydroxide (NALC-NaOH) procedure [ 9], then inoculated on two slants of Lowenstein-Jensen medincubatedated at 37°C.
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