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The rapid-salinity-decrease experiment lasted for 140 h, and the survival of specimens was checked 28 times in total in a similar manner to the gradual-salinity-decrease experiment.
Quality control for temperature transporting specimens was checked and confirmed; i.e., 4 9 degree Celsius.
Each of the specimens was checked for its label, quantity and procedure of collection.
After arrival at the central service unit completeness of the number and kind of specimens was checked and specimens were stored at -80°C.
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A total of 27 specimens were checked.
All tumor specimens were checked by HE stain for tumor content before analyses.
The stainings of TNFSF4 in renal biopsy specimens were checked by immunohistochemistry.
Specimens were checked every 24 h, the stones and the tissue wetted and fresh food added whenever necessary.
When CB was guided by stereotaxis, five passes were routinely undertaken, and the specimens were checked for calcification by radiography if appropriate.
Extracted DNAs from tissue specimens were checked for their qualities and the absence of PCR inhibitors by PCR for β-globin (Khan et al, 2008).
The current supplied to each specimen was checked on a regular basis to correct any drift.
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