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Genomic DNA was prepared from whole blood specimens using standard procedures.
Genomic DNA was prepared from tail specimens using standard procedures [33].
DNA was extracted from the obtained specimens using standard methods as were isolation of lymphoblastoid cells from whole blood.
For aCGH, high quality genomic DNA (0.5 to 3 µg) was isolated from frozen tumor specimens using standard methods and digested with restriction endonucleases AluI and RsaI according to the Agilent Oligonucleotide Array-based CGH for Genomic DNA Analysis Version 4.0 protocol.
Genomic DNA was extracted from surgical specimens using standard techniques.
DNA was isolated from blood specimens using standard methods.
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Venom was extracted from the specimen using standard methods [ 79] (ketamine, 35 μg/g; pilocarpine, 6 μg/g).
Non-fasted blood specimens, taken using standard techniques, were spun and frozen to −80°C within 18 h.
The single specimen positive using standard media was also positive using the enhanced protocol; the enhanced technique therefore detected one additional isolate of B. pseudomallei compared to the standard protocol.
It has also been known that there is a difference in the constraint effect between real pipes and standard specimens, and LBB design using standard specimens is conservative.
Freshly collected specimens were identified using standard taxonomic references [ 35- 38], with subsequent confirmation through comparison with specimens in WIN, MMMN, and CAN.
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