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Hypermethylation was investigated at eight loci in 113 PCa specimens using published validated MS-PCR methods (Supplementary Table).
We also analyzed the association between FABP7 and EGFR expression in different sets of GBM specimens using published DNA microarray datasets and semi-quantitative immunohistochemistry.
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We used viral surveillance data to estimate the proportion of influenza-positive specimens if all had been tested by using published methods (9 ).
The developed formulation is numerically tested and benchmarked, being validated by using published experimental results obtained from sandwich specimens.
Species identities were determined using published descriptions (e.g. [ 16]), the most recent morphological keys (e.g. [ 16, 22]), and descriptions of several new species [ 26] Specimens or portions of specimens were fixed in 80-100% ethanol by their respective sources.
During the preparation of this manuscript Fassan et al. published the microRNA expression profiling of 23 male breast cancer specimens using a published microarray platform [ 5].
Be sure to use published work only.
See Figure 5 for an example showing specimens used in a published research article.
The development of these methods – known as the crack modelling method and the theory of critical distances – is described, summarising previously published work on test specimens using steels and aluminium alloys.
Subchondral bone histomorphometry was performed on three nonconsecutive sections of each specimen using our previously published method [ 18], modified from that of Matsui and coworkers [ 19].
If lacking a published description, they were assessed from the sampled specimens using a Zeiss StereoDiscovery microscope with Zeiss AxioVision software.
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