If ribosomal DNA copies within a specimen are more diverse than between specimens, phylogenetic analyses using one representative sequence for each sampled specimen may not accurately represent relationships by descent.
Mortar and concrete specimens were made using one alkali-bearing reactive aggregate, one alkali-free reactive aggregate and one alkali-free non-reactive aggregate in order to determine the effect of an alkali release by an aggregate into the pore solution on the development of ASR using pore water extraction and direct expansion measurements.
All specimens were fixed using one 7.0-mm iliosacral screw into the S1 body.
Using fluoroscopic guidance, each specimen was then fixed using one standard-length 7.0-mm stainless steel cancellous fully threaded cannulated iliosacral screw (Zimmer, Inc., Warsaw, IN, USA) into the S1 vertebral body.
Further analysis was therefore conducted using one specimen of each Alloteropsis taxon together with other species resolved in the x = 9 clade.
The OECD 313 experiments were performed using one test specimen from this batch, but mainly using test specimens of 5 × 2.5 × 1.5 cm3 as defined in EN 113 [26] that were vacuum-impregnated with the same preservative.
Four true positive but discordant specimens were blocking assay negative using one or both EIA kits.
Therefore, once the CZM parameters have been defined for a given solder system using one BGA specimen, the CZM could predict the fracture of BGA solder joints with different geometries and strengths.
Expression levels of p53, VEGF-A, VEGF-C, MMP-7, HER2, Reg IV, olfactomedin 4 and Claudin-18, were analyzed by immunostaining using one representative specimen from the center of the tumor.
To confirm the taxonomic determination of the examined specimens, molecular phylogenetic trees were constructed using one mitochondrial gene, cytochrome c oxidase subunit 1 (COI) and one nuclear marker, the internal transcribed spacer 2 (ITS2).
