Sentence examples for specimens used previously from inspiring English sources

Needle biopsy specimens used previously were not included in this study because of insufficient tissue for immunohistochemical staining.

We have used previously analyzed cervical cytobrush specimens that were selected as either negative or positive controls.

For the positive controls, we used previously known MMP-2-positive specimens of breast carcinoma.

Although the specimen blocks used for the present study were the same as those used previously, the improvement in detail is striking.

Primary osteoblast cultures were obtained from bone specimens using a previously described technique [ 22] slightly modified for our experiments.

The immunoreactivity for IL-6 and TGF-β1 in the lung cancer specimens was quantified using previously validated procedures [41], [42]: no staining (grade 0), weak but detectable focal staining (grade 1), moderate staining (grade 2), marked staining (grade 3), very intense staining of the tumour cells (grade 4).

In addition, 32 primary acute myeloid leukaemia specimens used for methylation analysis were described previously (Toyota et al, 2001).

A description of the normal human liver tissue specimens used for these studies was presented previously [ 36].

Total RNA samples from frozen prostate tumour specimens used in this study were prepared previously (Nam et al, 2007).

Rotavirus positive specimens were then genotyped using previously described methods to determine the infecting strain.

The specimens used in this study were collected from the Tejo River Basin RiverRaiaias, as previously described [15].