Species identification and/or classification from a single cell has an ultimate value for various purposes: genome analysis of precious specimens using only a minute fraction of the available sample or tracing alterations in the genome of somatic cells (e.g., differences between the genomes of the cells from the brain and heart).
In order to guarantee comparability between specimens we used only skins of adult male specimens in spring or summer plumage collected from breeding areas.
Glioma tissue paraffin specimens and U87 glioma cell transplantation tumor specimens were used only in the laboratory.
The results of both obtained specimens are used only in Table 2 where we directly compare them to those of only one specimen.
For immunohistochemical evaluation of all clinical specimens, we used only the mouse monoclonal antibody (mouse IgG1, clone 44, BD Transduction Laboratories™, Europe), which had been previously used successfully [ 10, 12, 18, 20].
The Bedouin were from the two traditional lineages Adnani: Mutran (n=30), Aniza (n= 21) and Awazim (n= 37)—and Qahtani: Ajman (n=39), Shimar (n= 21) and Murrah (n= 5; because of the small sample size, Murrah specimens were used only in the autosomal analysis).
For immunohistochemical evaluation of all 97 clinical specimen, we used only the monoclonal antibody (clone MOG/07), which has been successfully approved before (Weichert et al, 2004; Mezzanzanica et al, 2008).
We used only specimens that had been shown previously to contain authentic endogenous DNA [ 19, 23, 24, 33].
For immunohistochemical evaluation of all 97 clinical specimens against ADAM17, we used only the polyclonal antibody, as it displayed a more intense staining warranting a more diligent analysis.
To evaluate the accuracy of smear microscopy for detecting M. tuberculosis, except one study [ 5] in which patients provided two sputum specimens each, all studies used only a single sputum sample.
