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The lack of proof-reading activity during the PCR might have increased the error rate in the sequence data, although the numerous specimens used here as well as the sequence coverage obtained should adequately compensate for this.
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Originally, Gastrophrynoides was a monotypic genus but the specimen used here is a newly found species of this genus (G. immaculatus) [ 5].
Due to the probable complexities, a 3/10-scaled specimen is used here.
In order to quantify the effect that these mechanisms had on the energy dissipation process, R-curves were generated which suggested that the specimen configuration used here is appropriate for characterising fracture toughness at low proportions of mode II.
Serial dilutions of well-characterized cell lines have been shown to generate a range of percent ratios similar to leukemic specimens and were used here to establish that the multiplex assay can reproducibly detect BCR ABL1 across a broad linear dynamic range.
Note: During the publication process of this paper it has been shown that the Romanian specimens of L. reali used here as outgroup actually belong to a new cryptic species named Leptidea juvernica [ 69].
More details on neuronal density and its relation with clinical features can be found in our previous study on NTs expression [ 9], since we used here the same specimens' series.
40 NGF immunostaining has been difficult to obtain in gut specimens, especially mucosal biopsies as we have used here.
The methods used here to reveal hidden features of the specimen may be applicable to other specimens from the fossil beds of Liaoning that produced Microraptor.
PTA is a much larger molecule [ 26], and the solution used here was found to require overnight incubation to penetrate specimens 2 3 mm thick, and longer for larger specimens.
It has been generally assumed that for respiratory infections due to viruses, the optimal specimen is the nasopharyngeal aspirate, rather than throat swabs we used here [ 16].
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