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This study was undertaken to evaluate the feasibility of using fluid-based Papanicolaou specimens to detect HPV using both the HC II and PCR methods in Japan.
We used 16S rRNA gene analysis of surgical tissues and culture of blood and tissue specimens to detect pathogens (data not shown).
However, our current study was limited by the lack of available serially collected specimens (to detect recurrence as in Friedman et al., 2012) at time of study design.
Although collection of NP specimens with a flocked swab is one of the best methods for obtaining specimens to detect influenza, those specimens might have been improperly collected by the nurses.
In addition to (or instead of) participating in the national surveillance, state laboratories in California, Maine, Maryland, Minnesota, New York, Texas, and Washington performed pyrosequencing on state surveillance specimens to detect the H275Y substitution.
As Spanish moss has a broad geographic range, we examined herbarium specimens to detect and characterize flowering/fruiting periods for the species across the Americas; we used high-temporal-resolution climatic data to assess the availability of optimal conditions for Spanish moss populations during each population's flowering period.
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Three duplex real-time reverse transcriptase-PCR assays were run on each specimen to detect matrix gene targets specific for influenza A and influenza B, hemaaglutinin gene targets specific for influenza A subtypes H1 (seasonal), H1 (pandemic) and H3, and MS2 RNA coliphage (MS2) to monitor the efficiency of the assay [S. Williams, G. Chidlow, D.W. Smith, manuscript submitted].
The ideal specimen to detect viruses depends on the type of assay being performed.
An immunohistochemistry study was performed on the liver biopsy specimen to detect CMV[ 1], but remained negative.
On the first day within 24 hours of admission, we collected the first serum specimen to detect the differences of TBI patients' LGT proteome abundance values.
Different solid media (e.g. selective agar plates containing antibiotics and substances favouring the growth of the sought microorganism) are inoculated with a stool specimen to detect and isolate enteric pathogens.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com