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Analyses were based on those specimens that resulted in detection of MDR Gram-negative organism.
The specimens that resulted positive were further tested by quantitative method (Light Cycler Instrument, Roche Molecular Biochemicals, Mannheim, Germany) to determine the viral load.
The specimens that resulted positive were further tested by a quantitative method (Cobas Amplicor HBV Monitor, Roche Molecular Systems, Branchburg, NJ, USA) to determine the viral load.
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This major gap was mainly explained by incorrect sputum specimen collection that resulted in salivary contamination (monthly mean rate 13.2%, range: 5.3% - 23.9%).
A Bohemian invention of the 18th century, cameo incrustation was taken up in Paris but had no vogue until Apsley Pellatt, an English glassmaker, developed a technique that resulted in specimens of genuine beauty.
To validate this approach, experiments were conducted on notched tensile specimens with varying notch-tip radii that result in a range of behaviour from stable to unstable damage growth as this radius increases.
Initial RT-PCR and nested PCR were performed with pooled tissue samples of individual vertebrates; when possible, for specimens that produced positive results, all tissues were retested separately.
Specimens that showed negative results in this first round of testing were further tested by using cultures, multiplex PCR, and resequencing pathogen microarrays.
In addition, in 2004 the definition for case patients was broadened to include patients with culture-negative specimens that yielded positive results by latex agglutination and Gram stain microscopy or by latex agglutination and polymerase chain reaction.
TOSV sequences were detected in 85 of 104 CSF specimens that provided positive results for viral sequence; However, 173 CSF specimens were negative by polymerase chain reaction (PCR); therefore, TOSV sequences were detected in 30% of the patients admitted for meningitis and in 40% of the patients admitted from June to November.
In contrast, PCR/ESI-MS reported a BSI-relevant organism in 173 additional specimens that were culture negative, resulting in a calculated assay specificity of 69% (Table S2, Supplemental Digital Content 1, http://links.lww.com/CCM/B418).lww.com/CCM/B418
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