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Each of the three approaches use in this study gave subtly different results in terms of the localisation of expression and the proportions of specimens that express Oct-6.
Finally, we identified vascular channels in necrotic areas of primary human high-grade invasive breast cancer specimens that express high levels of COX-2, suggesting that these channels may serve as an alternative means of generating microcirculation in hypoxic regions of the tumor and thus facilitate metastasis.
We evaluated the clonal IGHV segments between functionally defined regions of the IGHV genes: the highly conserved framework regions (FR1-3) which flank the hypermutated complementarity determining regions (CDR1-2) [ 37] for four FL specimens that express varying levels of mutational loads in their total IGHV sequence (10.3 to 17.9% variation).
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Finally, vascular channels were analyzed in surgically resected human breast cancer specimens that expressed varying levels of COX-2.
Human high-grade invasive tumor specimens that expressed high levels of COX-2 proteins had detectable vascular channels, whereas low-grade tumors with no or low COX-2 expression had little evidence of VM.
Of the 75 specimens that expressed both hPTTG1 and CXCR2 at high levels (hPTTG1: 2+/3+, CXCR2 2+/3+), 41 displayed strong (3+) or moderate (2+) p21 nuclear staining (41 (54.67%) of 75), and 34 (45.33%) demonstrated weak p21 expression.
Staining of specimens that moderately express HER2 protein may therefore be affected more easily by the type of fixative used compared to staining of specimens with high expression of HER2.
Normal bronchial tissue specimens that moderately expressed MUC5B were prepared as positive controls in all cases.
Normal gastric mucosa tissue specimens that moderately expressed MUC5AC were prepared as positive controls in all cases.
Of the nine specimens that weakly expressed both hPTTG1 and CXCR2 (hPTTG1+/0+/0+; CXCR2, 1+/0+), seven also displayed weak staining of p21.
In addition, our immunohistochemical findings of reduced expression of AR in advanced prostate cancer specimens especially those that express C/EBPα is in agreement with recent demonstrations of altered AR expression especially in androgen-independent prostate cancer [ 44].
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