The APT ToF mass spectra collected under voltage-pulsing mode are also qualitatively similar for both the IBAD (Fig. 2b) and the EBAD (Fig. 2c) specimens, although the EBAD specimens ran slightly longer before fracture.
These differences indicate that specimens analyzed in the extracted form are more readily detected than specimens run in unextracted form.
For treated mice, values of k c for specimens run immediately were 0.37 ± 0.05 (n = 3, a 68% increase, p = 0.015) and for specimens incubated in vitro before the run, 0.17 ± 0.03 (n = 3, a 54% less, p = 0.005).
Swab specimens (run in duplicate) with cycle threshold (Ct) values <35 were considered positive for influenza Avirus; specimens with Ct values of 35 to <40 were suspected to be positive; and specimens with Ct values >40 were considered negative.
The Clinical Laboratory Standards Institute (CLSI) evaluation protocol for a qualitative diagnostic test recommends analysis of a minimum of 50 positive and 50 negative specimens run over 10 20 days, in the absence of an existing body of validation data.
All TEG specimens were run on Thromboelastograph Analyzer 5000 (Haemoscope Corporation, Braintree, MA, USA).
GC weighed and measured the specimens and ran the statistical analyses.
All specimens were run in batches that included blanks, a standard calibration curve, two spiked specimens, and one duplicate.
All specimens were run in batches that included a sample blank, a standard calibration curve, and two spiked specimens each time.
At each step, every well contained 50 μl; specimens were run in duplicate in each experiment, and two or three experiments were performed with each plasma/peptide combination.
