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However, extant A. lessonii specimens require a winter isotherm of 14 °C, and are restricted to the southern coasts of the Mediterranean Sea where they thrive under tropical summer SSTs (Langer and Hottinger, 2000).
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As the results, the finer sand grading specimens required a higher water to cement ratio to achieve an equivalent workability.
The reduced shear span-to-depth ratio in the two-point load specimens as compared to the single point loading specimens required a higher load, and thus higher shear force, to develop the plastic hinges.
Reproduction of the medium resolution diffraction from our dehydrated specimens required a two-protofilament model, suggesting that dehydration may induce the close association of fibrils.
Furthermore, new species require a specimen as the type, and new observations often require microscopic study of specimens or careful quantitative measurements.
The major advantages of cryo-EM are that the specimens require no crystallization and are embedded in a thin vitrified ice of buffer thus preserving their near-native structure.
Effective studies of clinical specimens require low-volume assays, high precision measurements, and the ability to process many samples.
According to the literature, arcan specimens can be used, but bonded specimens require complex test assemblies and are costly in manufacturing.
The results are promising however the specimens require two bonding processes, one for the adhesive being tested and one extra for the backing adhesive.
A CT scan with a 1 mm slice thickness was made of all specimens required and a 3D reconstruction was generated (Institute for Diagnostic and Interventional Radiology, Düsseldorf University Hospital, Germany).
Laboratory culture work requires biosafety-level 3 (BSL-3) conditions and primary cultivation from a clinical specimen may require a seven-day incubation before colonies visible to the naked eye appear.
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