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Positive and negative controls were performed using specimens previously validated.
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In addition, if plate counts did not fall within 1 SD of the average of previously validated specimens, the corresponding assay was rejected and re-tested.
Liver biopsy specimens were evaluated according to the previously validated Metavir scoring system.
Piconi et al. reported a diminution in carotid intima media thickness (cIMT; an established, non-invasive measure of atherosclerotic burden previously validated against histology specimens.
The serum specimens were first screened by using an SBV indirect ELISA (i-ELISA) that was previously validated for the serum specimens from cattle, sheep, and goats (ELISA ID Screen Schmallenberg Virus Indirect, Bicupule; ID Vet, Montpellier, France) (7 ).
Nose and nappy specimens are batch tested for viruses using previously validated and reported real-time PCR assays, or PCR assays developed specifically for this study (table 1).
Mechanical behavior of the retrieved components was determined using the previously validated small punch test, which subjects miniature specimens to multi-axial loading conditions.
The NMCSD laboratory previously validated the APTIMA Combo2 Assay (Gen-Probe Inc., San Diego, California, USA) for testing the pharyngeal and rectal specimens according to Clinical Laboratory Improvement Amendments (CLIA) standard Sec 493.1253.
Specific curricula have been previously validated.
We constructed variables from previously validated instruments when available.
The 20 items were selected from previously validated depression scales.
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