Sentence examples for specimens or cell from inspiring English sources
Previous studies detected no KRAS mutations in NPC specimens or cell lines [ 29, 40].
Breast tissue specimens or cell pellets were homogenised in 1 ml TRIzol® lysis reagent (Invitrogen) as previously described [ 27].
Total cellular RNA was isolated from tissue specimens or cell lines using the Trizol reagent (TaKaRa, Dalian, China), according to the manufacturer's instructions.
To investigate CSC properties such as tumor initiation and recurrence, many studies have examined methods for isolation of CSCs from cancer specimens or cell lines.
RNA was isolated from viably frozen specimens or cell lines growing in logarithmic phase using RNAqueous-4 PCR kits (Ambion corp, AM1914).
Genomic DNA was extracted from 30 pairs of gastric cancer tissues, 24 benign gastric mucosa with intestinal metaplasia, and 10 normal gastric specimens or cell lines by a DNA mini kit (Qiagen, Valencia, CA, USA).
Total RNA was extracted from breast tissue specimens or cells using the Trizol reagent (Invitrogen) following manufacturer's instructions.
This technology enables high-throughput molecular analyses of hundreds of tissue specimens or cells in a single experiment.
Total RNA was isolated from tissue specimens or cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol.
For DNA extraction, microdissected clinical specimens or cells harvested from CRC cell lines were digested overnight at 42°C (digestion buffer: 10 m M Tris-HCl [pH8], 1 m M EDTA, 1% Tween 20, 0.5% Proteinase K) then heated to 95°C for 10 min.
* NB stage was evaluated according to INSS criteria Approximately 80 clones per specimen or cell line were sequenced.
