However, unlike the FTIR spectra and compared to the signals obtained for the control compounds (Fig. 14J L), there was a clear dampening of signals seen with all the specimens of protein-NLP, calcium granules, and NB a result that could be due to the presence (and interference) of proteins in the particle scaffold, a possibility that was pointed out earlier [3].
The use of these human tissue specimens for protein expression studies was approved by the local ethics committee of the University Hospital of Cologne, informed consent was obtained from the patients, and the specimens were procured from the centralized Biobank of the Center for Integrated Oncology CIO Cologne-Bonnnn (http://www.cio-koeln-bonn.de/en/medical-professionals/biobank/).
Homogenisation of tissue specimens and determination of protein concentrations were performed as described previously (Sier et al, 1996).
Body weight and food and water intake were recorded every 7 days, while 24-hour urine specimen for measurement of protein excretion was collected on days 0 and 20 of pregnancy.
Listed are percentages of specimens with high protein levels based on a total of 26 PDAC (T specimens) and 25 normal ducts (D and Dt specimens).
The proteomic analysis performed in 4-day-old mice cerebellar specimens allowed the identification of protein changes associated with early bilirubin-induced neuronal damage.
As compared to other methods, electron microscopy is uniquely suitable for analysis of a broad range of specimens, from small proteins to large complexes.
The ERAP1-349 mutation was not found in any of the specimens with ERAP1 protein downregulation (data not shown).
This technique has allowed us the processing of a large number of tissue specimens for a wide range of protein determinations.
The objectives of the present work were to evaluate the morphological expression and clinical relevance of several MMPs and TIMPs of biological importance in invasive ductal carcinomas of the breast, by using the tissue microarray (TMA) technique, which has allowed us to process a large number of tissue specimens for a wide range of protein determinations (Kononen et al, 1998; Camp et al, 2000).
Finally, the prepared specimens of SAM with immobilized protein were stored in PBS solution at 4 °C before use.
