Specimens of heart, eye and the associated infraorbital sinus, central nervous system, lungs, kidney, spleen, liver and gonads (ovaries and testes) were collected and placed in 10% buffered formalin during autopsy, and were treated using a routine histological technique and embedded in paraffin.
The collection now includes 22,000 specimens of hearts, heart valves, lungs and blood vessels, many preserved in formalin in plastic bags.
H&E, hematoxylin and eosin; SCC, squamous cell carcinoma Fig. 2 Autopsy specimen of the heart in macroscopic findings (a): Cardiac tumor was 10 × 7 × 7 cm, occupied 70%% of the right ventricle.
The medical students of the second year will be first introduced to the normal anatomical and histological structure of the heart, then allowed to visualize and examine the specimens of the cadaveric heart both macroscopically and microscopically.
Specimens of all organs (heart, lung, liver, spleen, kidneys, small intestine, large intestine, mesenterial lymphnode, brain) were fixed in 7% neutral buffered formalin, processed, embedded in paraffin wax, cut at 3 µm, mounted on glass slides and stained with haematoxylin and eosin (H&E) according to standard procedures.
On the other hand, PCR of heart specimens was more often positive than culture of bladder wall and heart muscle [ 30].
Mouse heart ECs (MHECs) were isolated from the heart specimens of Tet-ON and Tet-OFF animals, as previously described [ 10].
Fig. 1 Nitrotyrosine staining of heart specimen collected at the end of the experiment after 24 h. a Two representative histological images of sham (left) and septic (right) animals (×10 magnification).
Borrelia miyamotoi DNA was detected in 1/251 (0.4 %) heart specimens of Apodemus flavicollis in the retrospective part of the study and in 1/46 (2.2 %) heart specimens of Apodemus flavicollis in the prospective part of the study.
The ΔdbpAB/dbpA'/1 and ΔdbpAB/dbpA'/2 bacteria were grown from 6 joint, 4 remote skin and 2 heart specimens of 6 examined mice at 1 week, and from 6 joint, 5 remote skin and 3 heart specimens of 6 mice at 2 weeks; all sites became culture-positive at 3 weeks.
According to current modified Duke criteria (20 ) before the examination of heart valve specimens, only 1 (3.6%) patient met the criteria for endocarditis and 17 (60.7%) met the criteria for possible endocarditis (Technical Appendix Table 1) All 28 patients underwent heart valve replacement because of valve damage (21 ).
