The first set of analyses was conducted to compare different specimens of freshly fixed sea urchins.
The size and location of the anatomical features varied slightly, but this had been observed among different specimens of freshly fixed animals as well.
Despite these problems, however, a large number of fixed specimens are held in collections across the world, and the human and pathogen genetic information they contain is often critical to important health-related investigations.
In the previous report, they used a limited number of fixed specimens for observation of spermatogenesis.
Antigen retrieval of fixed specimens employed 10 mM citrate buffer pH 6 for 20 minutes at 95°C.
Influenza virus gene sequences from a number of fixed specimens of wild birds collected circa 1918 show little difference from avian viruses isolated today, indicating that avian viruses likely undergo little antigenic change in their natural hosts even over long periods (24, 25 ).
We used histopathologic classification of fixed tissues specimens as final reference, as usually done in similar circumstances [24], [25].
Despite its many benefits, diffusion MRI of fixed ex vivo specimens also presents several unique challenges.
This is a cross-sectional and retrospective study, in which all cases diagnosed between January 2005 and July 2011 1,1377 abnormal specimens from fixed tissue sections of biopsies and LEEPs) were selected.
Semithin sections of specimens fixed by glutaraldehyde were stained with Richardson solution, while specimens fixed by glutaraldehyde including tannic acid were analyzed without further staining.
