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Accordingly, the implementation of ISH, in combination with IHC for the detection of clinically important miRNAs and protein markers in fixed specimens, now provides a fluorescence-based multicolor ISH/IHC assay.
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A single immunostaining or in situ hybridization reaction now provides information on all of the specimens on the slide, while subsequent sections can be analysed with other probes or antibodies.
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These technical advances now provide the opportunity to contribute molecular information with high chemical and spatial specificity at sufficient throughput to aid in the histopathological evaluation of patient specimens.
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