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Three candidate genes, β1 integrin, FGFR2 and WNT9a showed no difference in expression pattern between control and immobilised specimens (not shown).
Large nerve bundles were present in the spleen sections from 7 animals and weak PrPC immunolabelling of nerves was detected in the spleen of 5 animals (from 3 inoculated sheep and 2 control sheep in frozen spleen specimens, not shown).
Immunostaining of adhering cells showed a predominant cell membrane p185 expression; immunohistochemistry of paraffin-embedded HCPC I showed a medium-high p185 expression (2+) in almost all cells (as compared with breast carcinoma specimens, not shown, according to the manufacturer's instructions) (Dako Hercept Test, 2000).
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Very similarly, our preliminary data showed a relatively higher expression of GalNAc-T6 in primary colon cancer specimens than that in asynchronous liver metastatic specimens (data not shown).
Specimens not identified to element, such as non-diagnostic long bone or cranial fragments, and a subset of fragile specimens are not shown here.
Similar data was obtained from frozen unfixed liver specimens, (data not shown).
Growth of GAS in AF was not restricted to the serotype M28 strain, as serotype M1 strain MGAS5005 grew at a comparable density to the M28 strain in one of the individual growth-positive specimens (data not shown).
This semi-nested PCR protocol requires only 10 copies of cDNA template per sample to produce sufficient product for cloning and does not produce nonspecific product from original clinical specimens (data not shown).
Hierarchical clustering using the 39-gene signature and an ovarian cancer training dataset from M.D. Anderson Cancer Center with control/non-malignant and serous EOC revealed a highly similar pattern among patient specimens (data not shown).
Other MAbs did not show any specific immunostaining of tissue specimens (data not shown).
Only nonpathogenic bacteria were cultured from the respiratory specimens (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com