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When collected through primary care physicians, billing issues can be problematic, and each home visit for collection of blood specimens may require several hours of staff time.
Appropriate investigation is limited by the diagnostic techniques available; virus isolation from cerebrospinal fluid or the brain can delay a diagnosis because culturing is slow, sensitivity may be poor, and obtaining specimens may require special techniques.
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Laboratory culture work requires biosafety-level 3 (BSL-3) conditions and primary cultivation from a clinical specimen may require a seven-day incubation before colonies visible to the naked eye appear.
Furthermore, obtaining a positive specimen may require invasive techniques.
Especially troublesome specimens may need to be scrubbed a bit.
Heavily infected specimens may also require a trim to get rid of diseased parts.
In the absence of clinical response, the diagnosis may require surgical pathology specimens.
In conclusion, the identification of all organisms involved in polymicrobial invasive infections may require cultures of specimens from all accessible septic foci.
Thus, many different specimens may be required to produce a thorough plate for identification of a given species.
Multiple CSF specimens may be required.
For some species (e.g., mammals and birds), blood may be a source of genetic material, whereas whole specimens may be required to obtain sufficient quantities of DNA in others (e.g., amphibians and non-avian reptiles).
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CEO of Professional Science Editing for Scientists @ prosciediting.com