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Inhibition of miR-141, which is upregulated in NPC specimens, may affect cell cycle, apoptosis, cell growth, migration and invasion through targeting of BRD3, UBAP1 and PTEN.
However, this lack of significant association does not necessarily invalidate our hypothesis because the quality of the specimens may affect detection of some serotypes and, consequently, the result of the test.
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Specimen availability may be related to tumour size and patient outcome (Hoppin et al, 2002), and the quantity, quality, and preservation method of the specimen may affect feasibility of conducting certain assays.
For instance, the age and type of the specimen may affect test results, and cell lines made from primary tissue may exhibit chromosomal changes and mosaicism as an artifact of the transformation process.
Pyrometry should be avoided wherever possible because oxidation induced alterations of the specimen surface may affect the accuracy of measurement.
Regular team meetings are highly recommended and are intended in part to facilitate communication and to ensure the team is able to adapt to both major changes (such as a change in clinical development timeline or target indication) and minor changes (such as changes to the list of clinical sites or to the specimen collection method) that may affect assay development or validation.
The use of specimens from non-sterile sites may affect the accuracy of a test for identifying causative organisms; thus, even though we used strict criteria for determining the causes, one of the limitations of the current study was the use of sputum and urine to diagnose pneumococcal pneumonia.
There is little research into the acceptance of a blood specimen as opposed to a saliva specimen for rapid testing and this may affect compliance to testing.
The brain volume of some rare species was estimated based on few specimens (i.e., <3), which may affect the accuracy of the measures.
Delay to formalin fixation after specimen collection and extended fixation times may affect HER2 testing results.
Specifically, the environment surrounding diagnosis and collection of specimens from cases, such as surgical preparation, may affect blood chemistry in a way that introduces systematic changes that may be mistakenly attributed to the disease state.
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