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Microscopy and gram-staining of each pleural or CSF sample were performed 24 hours a day (Ziehl-Neelsen staining on demand), whereas bacterial culture on blood and chocolate agar plates was conducted only during working hours (specimens maintained at room temperature).
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When both a nasal and throat specimen were requested, specimens were maintained at 2 8 °C or on ice and all testing was initiated within 72 hours of collection.
Specimens were maintained at 25°C via a dual stage and objective heating unit (Tempcontrol 37-2 digital; Zeiss).
All tissue specimens were maintained at the Department of Pathology and patient medical records were available at Baystate Cancer Center.
Fresh frozen human vertebral specimens were maintained at −20°C until approximately 24 hours prior to testing.
Specimens were maintained at room temperature and processed within 72 h after collection.
After collection, specimens were maintained at room temperature for 30 60 minutes and centrifuged for 10 minutes at 4000 rpm; sera were stored at −80 °C until analysis.
Blood serum and urine specimens were maintained at 4 8 °C for two weeks and then moved to a storage facility where the urine was aliquoted into 4 vials (1.5 mL each).
Therefore, we included specimens stored in RNA later to determine whether the specimens maintained fidelity.
For each heating run, the specimen was maintained at the destination temperature for 15 min and then cooled to room temperature using a fan.
For each test, the specimen was maintained at a first rate temperature of 60″ and the final one of 30″.
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CEO of Professional Science Editing for Scientists @ prosciediting.com