Each of these goals requires the acquisition of large numbers of well-annotated clinical specimens with long term follow-up [19].
We believe 3SEQ could revolutionize the field of translational cancer genomics, by allowing investigators to perform gene expression profiling on large numbers of well-annotated archival tumor specimens with long term follow-up.
Since we did not have an adequate number of additional specimens with long term follow-up to test our predictive gene set, we turned to other published data sets.
VCCC, JWMT, WCN, DMKC, SSCC, and FHWC coordinated collection of clinical specimens in the long term care facilities.
Moreover, some specimens have been stiffened by bonding CFRP sheets to both sides, in order to study how their introduction may reduce the specimen deformability under long term loading.
If it turns out that there is significant variation over a life-span, and/or a low degree of genetic pre-determination then, for example, a child whose microbial sample was collected in neonatal life may not be identifiable from the specimen over the long term.
Six of the specimens were instrumented to monitor long term shrinkage strain development of the FRP-confined NSC and HSC, with three specimens allocated to each mix.
Therefore, suboptimal processing of CNB specimens or long-term fixation of surgically resected specimens might have been partly responsible for the difference.
This result may reflect inappropriate long term stored specimens (as a retrospective study), and/or the presence of mutations in the target regions of probes.
In certain clinical and research situations, performing such analyses on archival formalin fixed paraffin-embedded (FFPE) surgical specimens would be advantageous as large libraries of such specimens with long-term follow-up data are widely available.
