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PCR-based diagnosis of any form of extrapulmonary TB is challenging due to the paucibacillary nature of specimens, lack of adequate sample volumes, non-uniform distribution of bacteria in such specimens, presence of PCR inhibitors, and lack of proper gold standard for evaluation of its diagnostic potential [3].
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In one Vulpes vulpes specimen, classification was of 1.436 below our classification threshold for modern mammal (2.0); in another Vulpes vulpes specimen, lack of classification was due to a low quality of the MALDI-TOF MS spectra (Table S5).
These differences may be due in part to use of nonspecific antibodies to assay ER β and some studies of archival specimens lack validation of a true TNBC phenotype [ 17, 20, 21].
For the mitochondrial dataset only two of the 34 specimens lacked one of the five sequenced fragments.
Detection of F. tularensis in hare specimens, including bone marrow specimens, and lack of F. tularensis detection in samples of the water system used to rinse hares suggest infection of the hares.
The teeth of the Vellberg specimens lack the striations apparent in the Vinita specimen [ 14] and reported in Whitakersaurus, as well as the flanges or obvious wear facet of derived rhynchocephalians such as clevosaurs, eilenodontines, and sphenodontines [ 10, 136, 138].
Umbilicus is a small succulent herb, which is perhaps most likely to harbour cryptic diversity because it is an undercollected species, and most of the herbarium specimens lack important characters for identification.
These inconsistencies could be due to relatively small cohorts, technical variability in staining from specimen to specimen, and lack of quantitative measures for immunohistochemistry.
All of these specimens lacked the extremes of Asfc evinced by Lophocebus albigena and especially Cebus apella, both known to consume hard, brittle foods.
When contrasted against the geographical distinction between Papua New Guinean and Australian G. elegans (ssp. dietrichiae, endemic to Australia) specimens, the lack of micro-biogeographic structure detected in Australian ssp.
Analysis of DAPI-labeled nematodes indicated the expected presence of nuclei throughout many specimens yet lack of copious ectobionts.
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