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For POCTs to have maximum value in remote settings, the use of minimally invasive, easily obtained, processing-free specimens is optimal.
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We found that the undiluted saliva specimen was optimal.
No one disputes that quitting is optimal.
Having products nearby is optimal.
Three or less is optimal.
However, ironing both sides is optimal.
Geographic data for specimens is generally less than optimal and is unevenly distributed across taxa, due to the bias of collecting activities toward particular species or locations, a historically insufficient prioritization of recording and maintaining of geographic data, lack of high quality absence data for species, and limited accessibility of stored data for some collections.
Because microbiologic processing may not be available around the clock, optimal storage of specimens is essential for accurate microbiologic identification of pathogenetic bacteria.
The optimally orientated specimens were embedded in optimal cutting temperature compound (Tissue-Tek OCT; RA Lamb, Eastbourne, UK) and snap-frozen.
According to Shen et al., who analysed the stability of candidate miRs and small RNA reference genes in cervical cancer cell lines and other cervical specimens, miR-23a has proven to be optimal reference microRNA and was suggested to be used for normalization [ 18].
Then, the specimens were covered in Optimal Cutting Temperature compound (OCT, Tissue-Tek) and frozen at −80°C for at least one hour and kept frozen thereafter.
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