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The specimens is added to RIPA buffer (89900, Pierce Biotechnology, IL, USA) containing protease inhibitor cocktail (78410, Pierce), and sonication.
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For example, when long-term specimens were added in the year 2002, the BED estimate increased from 4.38 to 6.03.
Human cerebrospinal fluid (CSF) samples and mouse specimens were added to Vero cells.
The specimens were added to Madin-Darby canin kidney (MDCK) cells.
All 109 stool specimens were added to Robinson's medium for growth of Entamoeba species.
Specimens were added to a PreservCyt solution vial (Cytyc Corporation) and were allowed to stand in the vial for 15 minutes.
It was housed in a glass container above a sample of blotting paper to which the urine specimens were added.
The conjunctival specimens were added into 24-well culture plates, containing HeLa cells, and incubated at 37°C.
One hundred microliters of 1 10 diluted fecal specimens or 1 2 diluted urine specimens was added to wells previously coated with antinucleocapsid monoclonal antibodies.
To isolate influenza viruses, respiratory specimens were added to primary cynomolgus monkey kidney tissue cultures, which were rolled at 33°C and observed daily for cytopathic effects.
The specimens were added onto B95a cells (10 ) that had been seeded into 25-cm tissue culture flasks and observed daily for characteristic cytopathic effect (CPE).
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